An undergraduate research student wishes to create a system that mimics human blood (pH = 7.4). The student chose as the model system to use a buffer solution of sodium dihydrogen phosphate (NaH2PO4, Ka = 6.2 × 10–8) and sodium hydrogen phosphate (Na2HPO4, Ka = 4.2 × 10-13).
A) Which of the components of the buffer solution is the acid?
B) Using the Henderson Hasselbalch equation, calculate the mole ratio of Na2HPO4/NaH2PO4 the student needs to use.
C) To approximate the human condition of blood alkalosis (where the pH of blood rises to 7.6, which is usually lethal), how much base will the student need to add to the buffer?
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